Sanger's DNA Squencing
1.The primer used in Sanger sequencing
A) can have any nucleotide sequence
B) must have a sequence beginning and ending with the same nucleotide
C) has a nucleotide sequence complementary to the 3' end of the region to be copied
D) has a nucleotide sequence complementary to the 5' end of the region to be copied
2. To carry out Sanger sequencing a mixture is needed containing
A) single-stranded DNA
B) DNA polymerase
C) four deoxyribonucleotides A, T, C, G
D) all of the above
Q & A
check your understanding!
3. When a dideoxyribonucleotide is added to the tube
A) replication of the strand continues
B) replication of the strand stops
C) replication of the strand is not affected
D) replication of the strand is speeded up
4. To sequence DNA by this method, the DNA must first be made in single stranded form.
A)True
B)False
5. Eamining the electrophoresis gel and reading from bottom to top, one base at a time, gives the sequence of DNA.
A)True
B)False
Check your answer
1. C) The primer has a nucleotide sequence that is complementary to the 3' end of the region to be copied so that DNA polymerase can begin replication.
2. D) All of these components are needed.
3. B) When a dideoxyribonucleotide is added to the tube, replication of the strand stops.
4. A) The DNA must be obtained in single stranded form to act as a template.
5. A) The oligonucleotides migrate by size. Reading the gel from from bottom to top, one base at a time, gives the sequence of DNA.